Proteomics Facility


Single Molecule Array platform

Quanterix SIMOA HD-1

Our HD-1 analyzer is a fully automated instrument for running immunoassays using single molecule array technology (SIMOA), a digital form of ELISA, trapping and sealing individual immunocomplexes on paramagnetic beads in thousands of femtoliter sized wells in arrays found on the SIMOA discs. By leveraging the digital detection format, this technology enables to precisely measure protein biomarkers with substantially higher sensitivity allowing the application of precision science to precision health.

Main technical specifications

  • Throughput of 3 plates every 8 hour shift. About 300 data points per day.
  • Workflow, batch (plates or tubes)
  • Total assay time is less than 2.5 hours per 96-well plate
  • Hands-on time, startup time <20 minutes
  • Sample input, 96-well plate and tubes
  • Sample volume, 1 µl * - 100 µl
  • Multiplex capability, up to 4-plex
  • Assay flexibility, 1, 2 or 3-step assays, with variable incubation time and number of wash steps

This equipment is open to external users upon request.


Chromatography

Achucarro's Bio-Rad NGC Medium-Pressure Liquid Cromatography system

Our Proteomics Unit is equipped with a BIO-RAD Quest 10 Chromatography system that allows easy, automated and all-purpose purification of proteins and biomolecules. It has automated 10 ml/min pumps that provide accurate gradients for high-resolution separation of proteins and biomolecules. It also has a wavelength detector with monitoring for high accuracy detection of proteins combined with conductivity measurements. Besides It supports automated sample injection using dedicated loops, fractionated samples can be collected using its specific fraction collector.

Main technical specifications

  • F10 pump (flow rate 10 ml/min)
  • Sample pump (for automated sample application)
  • Mixer module (includes a mixer motor and integrated pressure sensor)
  • Single-wavelengh detector module (UV detection at 255 or 280 nm)
  • Conductivity detector module (includes temperature sensor)
  • Sample inject valve (μl to L volumes)
  • Air sensor (detects end of buffer and sample to protect against column damage)
  • Valves (sample inject valve, buffer blending valve, pH valve, column switching vavle and outlet valve)
  • BioFrac Fraction Collector (versatile capability for sample collection)

This equipment is open to external users upon request.