Imaging Platform | Optical Microscopy

CONFOCAL AND SUPER-RESOLUTION OPTICAL MICROSCOPY

Leica Stellaris 5

Our confocal microscope is a (vertical) Leica Stellaris 5 (DM6 B CS). The configuration of the excitation lasers includes the WLL2 that allows excitation lines in the range between 485 and 685nm with a selection precision of 1nm.

  • Objectives: HC PL APO 10x/0.40 Air CS2, HC PL APO 20x/0.75 Air CS2, PL APO 40x/1.30 Oil CS2, PL APO 63x/1.40 Oil CS2, PL APO 40x/1.10 Water CS2, HC APO L 40x/0.80 W U-V-I diping
  • Galvanometric Z-stage and motorized XY-stage for multiposition imaging (mosaic, time lapse).
  • Second generation White Light Laser (WLL2).
  • Ultraviolet Laser (405 nm excitation line, AOTF Flexible)
  • Second generation Hybrid detectors (x3)
  • Supercontinuum Fiber Laser:
    Excitation range between 485 – 685 nm. in 1nm steps.
    Pulsed laser. Pulse frequency: 80MHz (compatible with lifetime measurement applications for processing). Laser lines controlled by AOTF and AOBS that allow to vary power and the simultaneous use of up to 8 wavelengths simultaneously.
  • TauSense module
  • LIGHTNING super resolution module
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