The Femto-2D microscope allows fluorescence imaging of neurons in living brain slices and in vivo. In addition to imaging live cell morphologies, it can be used for calcium imaging and other functional fluorescence microscopy approaches.

The setup is based on a tunable femtosecond laser equipped with a DeepSee module to compensate pulse dispersion and facilitate imaging deeper inside tissue. GaAsP and PMT detectors, respectively, allows for dual color imaging. The upright microscope is additionally fitted with electrophysiology equipment for two channel field or whole-cell recordings, as well as an LED epifluorescence module.