The existence of in vivo neuroimaging methods such as positron emission tomography, single photon emission computed tomography, magnetic resonance imaging, computed tomography, optical and ultrasound imaging have been indispensable for early diagnosis, in monitoring disease progression and therapy of a wide range of brain diseases such as stroke and brain hemorrhage. The inflammatory response is a major factor in neurovascular pathophysiology and contributes to secondary neuronal damage in both acute and chronic stages of the ischemic injury. Recent work in experimental cerebral ischemia has demonstrated the involvement of neurotransmitter signaling in the modulation of neuroinflammation, providing evidence of the role of neurotransmission receptors as a promising inflammatory biomarkers in neurovascular research. Therefore, our main goal is to evaluate the therapeutic potential and diagnostic perspectives of cannabinoid, cholinergic, purinergic and glutamatergic receptors and transporters in experimental stroke using non-invasive molecular imaging technologies. Besides, we are also interested in to visualize and quantify biological processes (i) during acute phase of ischemic stroke and subarachnoid hemorrhage as metalloproteinase activity, oxidative stress, brain perfusion and cell death at the cellular/molecular level and (ii) affecting sub-acute phases as angiogenesis and brain function recovery.
This research will foster the emergence of new therapeutic strategies that may recognize and modulate the inflammatory response after neurovascular pathologies. Finally, the knowledge derived from this research could be easily applied to other neurologic and neurodegenerative diseases.
Murine models of stroke and subarachnoid hemorrhage, Positron emission tomography, Single photon emission computed tomography, Magnetic resonance imaging, Computed tomography, Tomographic optical imaging, Ultrafast ultrasound imaging, Autoradiography, Immunofluorescence, Epifluorescence and Confocal microscopy, Gel zymography.
Pharmacokinetic Evaluation of New Drugs Using a Multi-Labelling Approach and PET Imaging: Application to a Drug Candidate with Potential Application in Neuromuscular DisordersBiomedicines (Jan, 2023) DOI: 10.3390/biomedicines11020253
Assessment of Regional Lung Ventilation with Positron Emission Tomography Using the Radiofluorinated Gas [18F]SF6: Application to an Animal Model of Impaired VentilationMolecular Imaging and Biology (Sep, 2022) DOI: 10.1007/s11307-022-01773-7
Longitudinal evaluation of neuroinflammation and oxidative stress in a mouse model of Alzheimer disease using positron emission tomographyAlzheimer's Research & Therapy (Jun, 2022) DOI: 10.1186/s13195-022-01016-5
Endovascular administration of magnetized nanocarriers targeting brain delivery after strokeJournal of Cerebral Blood Flow & Metabolism (Feb, 2022) DOI: 10.1177/0271678X211028816
Opposite alterations of 5HT 2A receptor brain density in subjects with schizophrenia: relevance of radiotracers pharmacological profileTranslational Psychiatry (May, 2021) DOI: 10.1038/s41398-021-01430-7