We incorporate one Seahorse XFe96 Extracellular Flux Analyser
ACHUCARRO has just incorporated to the Cell Analytics Facility one Seahorse XFe96 – Extracellular Flux Analyzer able to simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis – in a microplate and in real-time.
This equipment can determine in vitro oxygen consumption rate (OCR), and extracellular acidification rate (ECAR), in order to assess cellular functions such as oxidative phosphorylation, glycolysis, and fatty acid oxidation.
The measurement of cellular bioenergetics on live cells enables time-resolved analysis and testing of multiple conditions per assay well. XF assays provide increased throughput and use less sample compared to conventional respirometry techniques. By incorporating automated compound addition and solid-state fluorescence sensors in a microplate format.
This equipment has received the financial support of the European Commission – European Regional Development Fund.