The main research interest of our laboratory is to understand the interactions between the endocannabinoid system with excitatory and inhibitory systems controlling neuronal circuits recruited in the physiological processing of high brain functions. Cannabinoid receptors play important roles in the functioning of the central nervous system, the normal and pathological brain ageing as well as in the development and initiation of abnormal behaviours and brain disorders. Therefore, our final goal is to gain knowledge about how disruption of neural circuits leads to disease conditions. In particular, we are focused on the molecular organization of the components integrating the endocannabinoid system, and on the study of the anatomical and functional interplays between this system and classical membrane receptors at excitatory and inhibitory synapses in key brain regions involved in stress, addiction and epilepsy. Also, we are committed to the comprehensive characterization of neural ion channel and receptor kinetics in the brain, by focusing on the anatomical and functional characterization of the cannabinoid CB1 receptors in astrocytes and in mitochondria (mtCB1R) of brain cells. Because of the importance of CB1 receptors, this knowledge is essential for obtaining a realistic and accurate model of the brain.
We have developed an interdisciplinary strategy combining electrophysiological, pharmacological and anatomical techniques applied to brain tissue of wild-type and genetically modified mice, and also to lesion-generated animal models of brain diseases.
The methods used and handled efficiently in the laboratory are: High resolution immunoelectron microscopy; Electrophysiology ex vivo in brain slices (extracellular field recordings and patch-clamp); Immunocytochemistry for conventional light and confocal microscopy; Tract-tracing; Behavioural tests; Organotypic cultures.
Omega-3 Recovers Cannabinoid 1 Receptor Expression in the Adult Mouse Brain after Adolescent Binge DrinkingInternational Journal of Molecular Sciences (Dec, 2023) DOI: 10.3390/ijms242417316
Altered glial expression of the cannabinoid 1 receptor in the subiculum of a mouse model of Alzheimer's diseaseGlia (Apr, 2023) DOI: 10.1002/glia.24312
Target cell-specific plasticity rules of NMDA receptor-mediated synaptic transmission in the hippocampusFrontiers in Cellular Neuroscience (Apr, 2023) DOI: 10.3389/fncel.2023.1068472
The effect of omega-3 fatty acids on alcohol-induced damageFrontiers in Nutrition (Apr, 2023)
Differential Subcellular Distribution and Release Dynamics of Cotransmitted Cholinergic and GABAergic Synaptic Inputs Modify Dopaminergic Neuronal ExcitabilityJournal of Neuroscience (Nov, 2022) DOI: 10.1523/JNEUROSCI.2514-21.2022
Up-regulation of CB1 cannabinoid receptors located at glutamatergic terminals in the medial prefrontal cortex of the obese Zucker ratFrontiers in Neuroanatomy (Oct, 2022) DOI: 10.3389/fnana.2022.1004702
GLAST versus GFAP as astroglial marker for the subcellular study of cannabinoid CB1 receptors in astrocytesHistochemistry and Cell Biology (Jul, 2022) DOI: 10.1007/s00418-022-02139-4
Cannabinoid CB2 Receptors Modulate Microglia Function and Amyloid Dynamics in a Mouse Model of Alzheimer’s DiseaseFrontiers in Pharmacology (Apr, 2022) DOI: 10.3389/fphar.2022.841766
Cannabinoid control of hippocampal functions: The where mattersThe FEBS Journal (Apr, 2022) DOI: 10.1111/febs.15907
The endocannabinoid system in glial cells and their profitable interactions to treat epilepsy: Evidence from animal modelsInternational Journal of Molecular Sciences (Dec, 2021) DOI: 10.3390/ijms222413231