Cellular membranes exhibit diverse molecular organisations, shapes, and dynamic behaviours, playing pivotal roles for cellular processes. Understanding the intricate relationship between membrane architecture and specific components is crucial for deciphering membrane-associated cellular mechanisms. Correlative light and electron microscopy (CLEM) emerges as a powerful tool, synergising fluorescence microscopy for component identification and temporal analysis with electron microscopy for high-resolution structural insights, enabling the precision localization of fluorescent signals within electron tomograms. In this talk, I will discuss the utility of CLEM in uncovering novel organelle remodelling mechanisms, as well as its potential in elucidating the subcellular underpinnings of neurodegenerative diseases.

More information:

• https://www.achucarro.org/laboratory/gtpases-and-neurosignalling/