{"id":6534,"date":"2022-07-13T11:24:13","date_gmt":"2022-07-13T11:24:13","guid":{"rendered":"https:\/\/www.achucarro.org\/?page_id=6534"},"modified":"2025-06-02T16:54:32","modified_gmt":"2025-06-02T14:54:32","slug":"molecular-biology-facility","status":"publish","type":"page","link":"https:\/\/www.achucarro.org\/es\/facilities\/molecular-biology-facility\/","title":{"rendered":"Molecular Biology Facility"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"6534\" class=\"elementor elementor-6534\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7d3276e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7d3276e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-c787429\" data-id=\"c787429\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-728ad3c elementor-widget elementor-widget-heading\" data-id=\"728ad3c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Molecular Biology Facility<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e1086b5 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e1086b5\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-7655f44\" data-id=\"7655f44\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a69f965 elementor-tabs-view-horizontal elementor-widget elementor-widget-tabs\" data-id=\"a69f965\" data-element_type=\"widget\" data-widget_type=\"tabs.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-tabs\">\n\t\t\t<div class=\"elementor-tabs-wrapper\" role=\"tablist\" >\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-1741\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"elementor-tab-content-1741\" aria-expanded=\"false\">CHROMATOGRAPHY<\/div>\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-1742\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-1742\" aria-expanded=\"false\">CORE EQUIPMENT<\/div>\n\t\t\t\t\t\t\t\t\t<div id=\"elementor-tab-title-1743\" class=\"elementor-tab-title elementor-tab-desktop-title\" aria-selected=\"false\" data-tab=\"3\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-1743\" aria-expanded=\"false\">SERVICES AND TECHNIQUES<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t\t<div class=\"elementor-tabs-content-wrapper\" role=\"tablist\" aria-orientation=\"vertical\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"true\" data-tab=\"1\" role=\"tab\" tabindex=\"0\" aria-controls=\"elementor-tab-content-1741\" aria-expanded=\"false\">CHROMATOGRAPHY<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1741\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"1\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-1741\" tabindex=\"0\" hidden=\"false\">Our <strong>BIO-RAD Quest 10 Chromatography system<\/strong> allows easy, automated and all-purpose purification of proteins and biomolecules. It has automated 10 ml\/min pumps that provide accurate gradients for high-resolution separation of proteins and biomolecules. It also has a wavelength detector with monitoring for high accuracy detection of proteins combined with conductivity measurements. Besides It supports automated sample injection using dedicated loops, fractionated samples can be collected using its specific fraction collector.\n\n<img decoding=\"async\" src=\"\/wp-content\/uploads\/2022\/07\/Facilities-images-cromatography.jpg\" alt=\"Bio-Rad NGC Medium-Pressure Liquid Cromatography system\" \/>\n<p>&nbsp;<\/p>\n\n<strong>Main technical specifications<\/strong>\n<ul>\n \t<li>F10 pump (flow rate 10 ml\/min)<\/li>\n \t<li>Sample pump (for automated sample application)<\/li>\n \t<li>Mixer module (includes a mixer motor and integrated pressure sensor)<\/li>\n \t<li>Single-wavelengh detector module (UV detection at 255 or 280 nm)<\/li>\n \t<li>Conductivity detector module (includes temperature sensor)<\/li>\n \t<li>Sample inject valve (\u03bcl to L volumes)<\/li>\n \t<li>Air sensor (detects end of buffer and sample to protect against column damage)<\/li>\n \t<li>Valves (sample inject valve, buffer blending valve, pH valve, column switching vavle and outlet valve)<\/li>\n \t<li>BioFrac Fraction Collector (versatile capability for sample collection)<\/li>\n<\/ul>\n<div style=\"background: #EFEFEF; padding: 10px;\"><a href=\"mailto:facilities@achucarro.org?subject=Achucarro%20Web%20%2F%20Facilities%3A%20Proteomics%20Unit\" target=\"_blank\" rel=\"noopener\">This equipment is open to external users upon request<\/a><\/div>\n<\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"false\" data-tab=\"2\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-1742\" aria-expanded=\"false\">CORE EQUIPMENT<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1742\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"2\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-1742\" tabindex=\"0\" hidden=\"hidden\"><p style=\"text-decoration: underline;\"><strong>ULTRACENTRIFUGE<\/strong><\/p>\nAs from 2024 we count with a HIMAC CP80NX Ultracentrifuge that can take up to 80.000rpm\/615.000xg (accuracy, \u00b110 rpm (1,000 rpm up to maximum), and two rotors, one of them oscilating (P32ST2) and the other fixed (P50A3).\n\nOther technical features:\n<ul>\n \t<li><span class=\"jCAhz ChMk0b\"><span class=\"ryNqvb\">Vacuum System: Dual system consisting of a rotary and diffuser pump<\/span><\/span><\/li>\n \t<li><span class=\"jCAhz ChMk0b\"><span class=\"ryNqvb\">Speed \u200b\u200bSetting Range: 1,000 rpm up to maximum speed<\/span><\/span> <span class=\"jCAhz ChMk0b\"><span class=\"ryNqvb\">in 100 rpm increments<\/span><\/span><\/li>\n \t<li>Temperature Setting Range: From 0\u00b0C to 40\u00b0C in 1\u00b0C increments (Accuracy: +\/- 0.5\u00b0C)<\/li>\n \t<li><span class=\"jCAhz ChMk0b\"><span class=\"ryNqvb\">Safety: EN61010-1 and EN 61010-2-020;<\/span><\/span> <span class=\"jCAhz ChMk0b\"><span class=\"ryNqvb\">Electromagnetic Compatibility: EN 61326-1<\/span><\/span><\/li>\n<\/ul>\n<table>\n<tbody>\n<tr>\n<td width=\"50%\"><img decoding=\"async\" src=\"https:\/\/www.achucarro.org\/wp-content\/uploads\/facilities_ultracentrifuge_himac.jpg\" \/><\/td>\n<td><img decoding=\"async\" src=\"https:\/\/www.achucarro.org\/wp-content\/uploads\/facilities_ultracentrifuge_himac_bfa.jpg\" \/><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"color: #000000;\"><strong>Bizkaiko Foru Aldundiak finantzatu du proiektu hau, 2023ko Teknologia Transferentzia Programaren barruan<\/strong> | \n<em>Este proyecto ha sido financiado por la Diputaci\u00f3n Foral de Bizkaia dentro del Programa Transferencia Tecnol\u00f3gica 2023<\/em> | \n<span style=\"color:#E97132\"><strong>This project has been funded by the Provincial Council of Bizkaia within the 2023 Technology Transfer Program<\/strong><\/span><\/p><\/div>\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-tab-title elementor-tab-mobile-title\" aria-selected=\"false\" data-tab=\"3\" role=\"tab\" tabindex=\"-1\" aria-controls=\"elementor-tab-content-1743\" aria-expanded=\"false\">SERVICES AND TECHNIQUES<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-1743\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"3\" role=\"tabpanel\" aria-labelledby=\"elementor-tab-title-1743\" tabindex=\"0\" hidden=\"hidden\"><p>The Molecular Biology Facility provides research support services to internal users.<\/p>\n<h4>Current services<\/h4>\n<p>Genotyping; RNA extraction;     qPCR, PCR; Purification of DNA; Enzymatic reactions and\/or ligation; Work with bacteria; and Cloning.<\/p>\n<img decoding=\"async\" src=\"\/wp-content\/uploads\/2022\/07\/Facilities-molecular-biology.jpg\" alt=\"Bolecular biology services and techniques\" \/>\n<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Molecular Biology Facility CHROMATOGRAPHY CORE EQUIPMENT SERVICES AND TECHNIQUES CHROMATOGRAPHY Our BIO-RAD Quest 10 Chromatography system allows easy, automated and all-purpose purification of proteins and biomolecules. It has automated 10 ml\/min pumps that provide accurate gradients for high-resolution separation of proteins and biomolecules. It also has a wavelength detector with monitoring for high accuracy detection [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":2319,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"set","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-6534","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.4 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Molecular Biology Facility - Achucarro Basque Center for Neuroscience<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.achucarro.org\/facilities\/molecular-biology-facility\/\" \/>\n<meta property=\"og:locale\" content=\"es_ES\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Molecular Biology Facility - Achucarro Basque Center for Neuroscience\" \/>\n<meta property=\"og:description\" content=\"Molecular Biology Facility CHROMATOGRAPHY CORE EQUIPMENT SERVICES AND TECHNIQUES CHROMATOGRAPHY Our BIO-RAD Quest 10 Chromatography system allows easy, automated and all-purpose purification of proteins and biomolecules. 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