{"id":5973,"date":"2022-05-02T13:33:08","date_gmt":"2022-05-02T11:33:08","guid":{"rendered":"https:\/\/www.achucarro.org\/facilities\/biomarkers\/"},"modified":"2026-03-09T10:30:54","modified_gmt":"2026-03-09T09:30:54","slug":"biomarkers","status":"publish","type":"page","link":"https:\/\/www.achucarro.org\/es\/facilities\/biomarkers\/","title":{"rendered":"Platform for Biomarkers"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"5973\" class=\"elementor elementor-5973 elementor-2368\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e72c628 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e72c628\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-60da812\" data-id=\"60da812\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1b06341 elementor-widget elementor-widget-heading\" data-id=\"1b06341\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Platform for Biomarkers<\/h2>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-0380bd5 facilities-tabs elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"0380bd5\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1ff5ec7\" data-id=\"1ff5ec7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-eba56c0 elementor-widget elementor-widget-text-editor\" data-id=\"eba56c0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>The Platform for Biomarkers offers <strong>three complementary immunoassay technologies<\/strong> to enable highly sensitive, multiplexed and robust detection of protein biomarkers across a wide dynamic range:<\/p><ol><li><strong>Single Molecule Array<\/strong> (SIMOA \u2013 Quanterix HD-X)<\/li><li><strong>Multiplex ultrasensitive immunoassays<\/strong> (Luminex\u00ae xMAP\u00ae \/ Intelliflex)<\/li><li>Standard <strong>high-quality ELISA assays<\/strong><\/li><\/ol><p>Each technology provides unique analytical capabilities, allowing users to tailor detection strategies according to the required sensitivity, sample volume, dynamic range, and number of analytes.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4bd1adc elementor-widget elementor-widget-heading\" data-id=\"4bd1adc\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">1. SINGLE MOLECULE ARRAY  (SIMOA) \u2013 Ultra sensitive digital immunoassays<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-53fad7e elementor-widget elementor-widget-text-editor\" data-id=\"53fad7e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Our <strong>Quanterix SIMOA HD-X analyzer<\/strong> is a fully automated platform based on <strong>Single Molecule Array (SIMOA)<\/strong> technology, a digital version of ELISA. Individual immunocomplexes formed on paramagnetic beads are isolated and sealed in thousands of femtoliter-sized microwells on SIMOA discs, enabling digital counting of single protein molecules.<\/p><p>This digital detection format provides <strong>up to 1000-fold higher sensitivity<\/strong> than conventional immunoassays, enabling precise quantification of extremely low-abundance protein biomarkers and supporting precision medicine applications.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ce32623 elementor-widget elementor-widget-image\" data-id=\"ce32623\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img fetchpriority=\"high\" decoding=\"async\" width=\"1120\" height=\"260\" src=\"https:\/\/www.achucarro.org\/wp-content\/uploads\/2022\/07\/biomarkers-simoa.jpg\" class=\"attachment-full size-full wp-image-6281\" alt=\"\" srcset=\"https:\/\/www.achucarro.org\/wp-content\/uploads\/2022\/07\/biomarkers-simoa.jpg 1120w, https:\/\/www.achucarro.org\/wp-content\/uploads\/2022\/07\/biomarkers-simoa-300x70.jpg 300w, https:\/\/www.achucarro.org\/wp-content\/uploads\/2022\/07\/biomarkers-simoa-1024x238.jpg 1024w, https:\/\/www.achucarro.org\/wp-content\/uploads\/2022\/07\/biomarkers-simoa-768x178.jpg 768w\" sizes=\"(max-width: 1120px) 100vw, 1120px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6fe3984 elementor-widget elementor-widget-text-editor\" data-id=\"6fe3984\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Main technical specifications<\/strong><\/p><ul><li>Throughput: 3 plates per 8-hour shift (~300 datapoints\/day)<\/li><li>Fully automated workflow (plate or tube format)<\/li><li>Total assay time &lt; 2.5 h per 96\u2011well plate<\/li><li>Hands\u2011on\/startup time &lt; 20 min<\/li><li>Sample input: 96\u2011well plate or tubes<\/li><li>Sample volume: <strong>1 \u00b5L\u2013100 \u00b5L<\/strong><\/li><li>Multiplexing: up to <strong>4\u2011plex<\/strong><\/li><\/ul><p><strong>Benefits<\/strong><\/p><ul><li><ul><li><strong>Ultra-sensitivity<\/strong>: detect proteins at fg\/mL levels<\/li><li><strong>Automation<\/strong>: highly reproducible, sample-to-answer workflow<\/li><li><strong>Multiplexing<\/strong>: up to 4 biomarkers in a single sample<\/li><li><strong>Flexibility<\/strong> for high-throughput or fast\u2011turnaround workflows<\/li><li><strong>Efficiency<\/strong>: stable calibration and consistent assay conditions<\/li><\/ul><strong>Broad menu<\/strong>: &gt;80 available assays + homebrew capability<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b4ffad6 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"b4ffad6\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6ddd5e0 elementor-widget elementor-widget-heading\" data-id=\"6ddd5e0\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">2. Luminex\u00ae INTELLIFLEX \u2013 Multiplex quantitative immunoassays<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a623b12 elementor-widget elementor-widget-text-editor\" data-id=\"a623b12\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>The platform incorporates <strong>Luminex\u00ae xMAP\u00ae \/ Intelliflex<\/strong> technology, enabling <strong>ultrasensitive multiplex detection<\/strong> of multiple analytes simultaneously using color-coded microspheres.<\/p><p>This method allows simultaneous quantification of up to <strong>50+ analytes<\/strong> in a single well, making it ideal for cytokine panels, signaling molecule profiling, and broad biomarker discovery studies.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a796aa4 elementor-widget elementor-widget-text-editor\" data-id=\"a796aa4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Key features<\/strong><\/p><ul><li><strong>High\u2011multiplexing<\/strong>: 10\u201350+ biomarkers per sample<\/li><li><strong>Low sample consumption<\/strong>: typically, 25\u201350 \u00b5L<\/li><li><strong>Flexible assay design<\/strong>: customizable multiplex panels<\/li><li><strong>Broad dynamic range<\/strong> and robust quantification<\/li><li style=\"list-style-type: none;\">\u00a0<\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2301a6b elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"2301a6b\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f11d51c elementor-widget elementor-widget-heading\" data-id=\"f11d51c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h3 class=\"elementor-heading-title elementor-size-default\">3. Standard ELISA \u2013 Robust single analyte quantification<\/h3>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ceac5d3 elementor-widget elementor-widget-text-editor\" data-id=\"ceac5d3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>The platform also offers <strong>classical ELISA<\/strong> assays, providing accessible, reliable, and cost-effective detection for single biomarkers.<\/p><p>Standard ELISA remains the gold standard for many clinical and research applications due to its <strong>high specificity<\/strong>, <strong>straightforward workflow<\/strong>, and <strong>excellent reproducibility<\/strong>.<\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d1a78e0 elementor-widget elementor-widget-text-editor\" data-id=\"d1a78e0\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><strong>Key features<\/strong><\/p><ul><li>High sensitivity and specificity for individual analytes<\/li><li>Ideal for validation of biomarker candidates<ul><li>Compatible with a wide variety of commercial kits<\/li><\/ul><\/li><\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1a0e700 elementor-align-center elementor-widget elementor-widget-button\" data-id=\"1a0e700\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t\t\t<a class=\"elementor-button elementor-button-link elementor-size-md\" href=\"\/wp-content\/uploads\/2022\/07\/Achucarro-Biomarkers-SIMOA.pdf\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t\t\t\t<span class=\"elementor-button-text\">Policy of use of the Achucarro Biomarkers services<\/span>\n\t\t\t\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-661c448 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"661c448\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-bd32976\" data-id=\"bd32976\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b6fc371 elementor-widget elementor-widget-heading\" data-id=\"b6fc371\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">SELECTED PUBLICATIONS OF THE PLATFORM<\/h5>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-90987aa elementor-widget elementor-widget-text-editor\" data-id=\"90987aa\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<ul>\n \t<li><strong><a href=\"https:\/\/doi.org\/10.1016\/j.redox.2026.104107\" target=\"_blank\" rel=\"noopener\">Hexaraphane as a potential therapeutic strategy for tauopathies<\/a><\/strong> Redox Biology, 2026. DOI: 10.1016\/j.redox.2026.104107<\/li>\n \t<li><a href=\"https:\/\/www.mdpi.com\/1422-0067\/26\/20\/10008\"><strong>Influence of Lifestyle on Brain Sensitivity to Circulating Insulin-like Growth Factor 1<\/strong><\/a> \u00a0<em>Mol Sci,<\/em> 2025. DOI: 10.3390\/ijms262010008.<\/li>\n \t<li><strong><a href=\"https:\/\/doi.org\/10.1038\/s41598-025-17394-3\" target=\"_blank\" rel=\"noopener\">Stepwise approach to alzheimer&#8217;s disease diagnosis in primary care using cognitive screening, risk factors, neuroimaging and plasma biomarkers<\/a><\/strong> <em>Scientific Reports<\/em>, 2025. DOI: 10.1038\/s41598-025-17394-3<\/li>\n \t<li><strong><a href=\"http:\/\/doi.org\/10.3390\/ijms25094594\" target=\"_blank\" rel=\"noopener\">Assessment of the Correlation and Diagnostic Accuracy between Cerebrospinal Fluid and Plasma Alzheimer\u2019s Disease Biomarkers: A Comparison of the Lumipulse and Simoa Platforms<\/a><\/strong> International Journal of Molecular Sciences, 2024. DOI: 10.3390\/ijms25094594<\/li>\n \t<li><a href=\"https:\/\/thejns.org\/view\/journals\/j-neurosurg\/138\/2\/article-p454.xml\"><strong>Serum assessment of traumatic axonal injury: the correlation of GFAP, t-Tau, UCH-L1, and NfL levels with diffusion tensor imaging metrics and its prognosis utility<\/strong><\/a> <em>J Neurosurg, 2023. DOI: 10.3171\/2022.5.JNS2263<\/em><em>8<\/em><\/li>\n \t<li><a href=\"https:\/\/doi.org\/10.3390\/ijms23169256\" target=\"_blank\" rel=\"noopener\"><strong>New, Fully Implantable Device for Selective Clearance of CSF-Target Molecules: Proof of Concept in a Murine Model of Alzheimer\u2019s Disease<\/strong><\/a> <em>International Journal of Molecular Sciences<\/em>, 2022. DOI: 10.3390\/ijms23169256<\/li>\n \t<li><a href=\"https:\/\/doi.org\/10.3389\/fnagi.2022.896848\" target=\"_blank\" rel=\"noopener\"><strong>Linking Plasma Amyloid Beta and Neurofilament Light Chain to Intracortical Myelin Content in Cognitively Normal Older Adults<\/strong><\/a> <em>Frontiers in\u00a0Aging Neuroscience<\/em>, 2022. DOI: 10.3389\/fnagi.2022.896848<\/li>\n \t<li><a href=\"https:\/\/doi.org\/10.1016\/j.parkreldis.2022.01.024\" target=\"_blank\" rel=\"noopener\"><strong>Characterization of molecular biomarkers in cerebrospinal fluid and serum of E46K-SNCA mutation carriers<\/strong><\/a> <em>Parkinsonism Relat Disord<\/em>, 2022. DOI: 10.1016\/j.parkreldis.2022.01.024<\/li>\n \t<li><a href=\"https:\/\/doi.org\/10.2147\/JIR.S334337\" target=\"_blank\" rel=\"noopener\"><strong>Whole Blood Expression Pattern of Inflammation and Redox Genes in Mild Alzheimer\u2019s Disease<\/strong><\/a> <em>Journal of Inflammation Research<\/em>, 2021. DOI: 10.2147\/JIR.S334337<\/li>\n<\/ul>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Platform for Biomarkers The Platform for Biomarkers offers three complementary immunoassay technologies to enable highly sensitive, multiplexed and robust detection of protein biomarkers across a wide dynamic range: Single Molecule Array (SIMOA \u2013 Quanterix HD-X) Multiplex ultrasensitive immunoassays (Luminex\u00ae xMAP\u00ae \/ Intelliflex) Standard high-quality ELISA assays Each technology provides unique analytical capabilities, allowing users to [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":5967,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"site-sidebar-layout":"no-sidebar","site-content-layout":"page-builder","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"disabled","ast-breadcrumbs-content":"","ast-featured-img":"disabled","footer-sml-layout":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"class_list":["post-5973","page","type-page","status-publish","hentry"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v26.4 - 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