Oligodendrocyte-derived exosomes in axon-glia interaction and glial support
University of Mainz (Germany)
Myelinating oligodendrocytes provide support to axons, which is incompletely understood. Extracellular Vesicles are released by cells and deliver molecular cargo to target cells. The presentation will highlight the transfer of oligodendrocyte-derived extracellular vesicles (exosomes) to neurons and provide evidence that glia-to neuron exosome transfer is important for neuronal homeostasis and maintenance. In the first part, I will demonstrate that glutamatergic signaling triggers the release of exosomes from oligodendrocytes and exosomes are endocytosed by neurons. In the second part, I will focus on recent results obtained with CreERT2-reporter mice and conditional deletion of genes associated with exosome biogenesis to genetically interfere with exosome secretion, providing proof of glia to neuron exosome transfer in different brain regions. Finally, I will discuss the functional role of oligodendroglial exosomes in axonal maintenance and show that oligodendroglial exosomes derived from mice affected by secondary axonal degeneration (PLP- and CNP-ko mice) lack the ability to support the neuronal metabolism and to promote axonal transport. Together, our work demonstrates that glia to neuron exosome transfer is a mode of glial support important for long-term axonal maintenance and integrity.
Currently we are hosting our seminar in Zoom. The link is available to external people on request.