Gliogenesis from the Subventricular zone after brain ischemia
Laboratory of Neurobiology, ACHUCARRO
Cell regeneration in adult mammalian brain can occur from the subventricular zone (SVZ) after an insult in terms of neurogenesis or gliogenesis. We previously demonstrated in vitro that neural progenitor cells (NPC) from the SVZ could generate fibrotic astrocytes expressing high levels of Thrombospondin 4 (Thbs4), after oxygen and glucose deprivation. In this study, we demonstrated that SVZ-NPC can generate mature astrocytes following brain ischemia using the middle cerebral artery occlusion as an experimental paradigm. After 15-30 days post ischemia, we observed by BrdU incorporation and electroporation cell labelling a significant increase of Thbs4+ astrocytes generated from the SVZ and migrating towards the ischemic scar (cortex and striatum). The role of newly generated astrocytes in modulating the extracellular matrix structure at the scar level. After immunofluorescence and ultrastructural image analysis of hyaluronic acid and mRNA expression of several matrisoma genes (Hyal1-3, Has1-2, and Mmps), we suggest that SVZ-derived astrocytes may have a role in remodeling the ischemic glial scar.
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