How top wrap the myelin
Universität Münster (Germany)
Rapid and efficient protocols to generate oligodendrocytes (OL) from human induced pluripotent stem cells (iPSC) may be a key technology to understand the biology of myelin diseases and to develop treatments for such disorders. The induction of three transcription factors (SOX10, OLIG2, NKX6.2) in iPSC-derived neural progenitor cells is sufficient to rapidly generate O4+ OL with an efficiency of up to 70% in 28 days and a global gene expression profile comparable to primary human OL. During my presentation I will demonstrate that these OL are suitable for in vitro myelination assays, the dissecting of mechanisms regulating oligodendroglial differentiation as well as d disease modelling.